Pompey Academy start their FA Youth Cup adventure with a trip to Gillingham on Tuesday evening.
The first round tie at Priestfield Stadium kicks-off at 7pm and must be decided on the night.
Entry to the game is priced for £4 for adults and £2 for concessions.
If Pompey progress then either Lewes or Camberley Town will visit Fratton Park in the second round.
To warm-up for the contest, the players took part in a crossbar challenge and you can watch the outcome below.