Pompey Ladies conceded a late goal to suffer an agonising defeat at title-chasing Charlton.
They created a plethora of chances against their high-flying opponents, only for Kit Graham to snatch a winner for the hosts in the 85th minute.
Shannon Albuery had an early opportunity for the Blues when she guided an Eilidh Currie free-kick just past the post.
Rachel Panting was then twice denied by Addicks stopper Katie Startup, who also did well to keep out Sammy Quayle’s strike.
Charlton looked much stronger after the break, however, and Graham fired a warning shot narrowly wide.
And it was Graham who ultimately proved the difference between the sides after converting a pass from Olivia Lukasewich.
"The passion, desire, work ethic and application was second to none."
The visitors still had a chance to ensure a share of the spoils, though, with Daisy McLachlan not quite able to beat Startup with a volleyed effort.
Blues boss Jay Sadler said: “It was not the result our performance deserved – the passion, desire, work ethic and application was second to none.”
Defender Ellie Kirby added: “It’s a hard result to take, but I’m so proud of how we played. We’ll learn from it and go again.”
Pompey: Levett; Kirby, Southgate (Perkins 50), Currie, Peck; Sievwright; Panting, Albuery, Boswell (c), McLachlan; Quayle
Sub not used: Maylin
There was also disappointment for the Blues’ second string, who were beaten 1-0 by Stoke in the final of the Premier League Reserve Cup.