Several first team players will be involved in the Pompey XI friendly against Gosport on Tuesday (7.30pm kick-off).
Christian Burgess, Louis Dennis, Brandon Haunstrup and Danny Rose are all in line to feature at Privett Park.
Third year scholars Matt Casey and Dan Smith – who have featured regularly throughout pre-season – are also expected to play.
The rest of the squad will be made up of members from Mark Kelly’s under-18 academy side.
Tickets can be bought on the turnstiles at Gosport, priced £10 for adults and £6 for seniors (65+), juniors (under 18) and disabled supporters.