Pompey Women suffered a second successive defeat, with a 2-1 National League loss to Coventry at the PMC Stadium.
Rachel Panting found the target to cancel out an early goal for the visitors in a dramatic opening to the contest.
But Coventry netted again and could afford a miss from the penalty spot as they went on to claim all three points.
Jay Sadler made four changes from the side that were beaten in the cup the previous week, with Bekah Tonks, Eilidh Currie, Sarah Eadon and Panting all coming into the side.
The Sky Blues were, likek Pompey, unbeaten in the league and travelled to the south coast expecting a tough test.
But it took the just five minutes to break the deadlock, as Helen Dermody tapped in a flick on at the far post.
It was originally ruled by the linesman, but – following a lengthy conversation – the referee awarded a goal, much to the disbelief of the hosts.
Pompey were back in the contest just four minutes later, however, with PANTING’s run down the right topped off with a rocket into the top corner of the net.
The officials were in the thick of it again midway through the first half, with the referee overruling his assistant’s decision to award the Blues a throw.
Coventry were instead allowed to play the ball into the box and Lois Jeffies teed-up Jade Brook to fire past Robyn Levett.
Pompey were still in a state of disbelief and had more misfortune when Amelia Southgate upended Jeffries in the box.
It was Dermody who stepped up to take the resulting penalty, only to send her effort narrowly wide of the post.
Half Time: Pompey 1 Coventry 2
The hosts began the second half knowing a change of luck was required and they created the better chances.
But a further blow was dealt when Shannon Albuery collided with team-mate Eadon and had to be substituted.
They almost levelled just past the hour mark, however, as Panting’s effort cannoned against the crossbar, while Hannah Geer sent the follow-up over.
Pompey remained on top for the remainder of the contest, but could not find a second goal as Coventry held on to claim all three points.
Pompey: Levett; Tonks, Southgate (c), Currie, Geer (Russell 76); Rowe, Perkins (Bradley 66), James; Panting, Albuery (McLachlan 58), Eadon
Subs not used: Kirby, Carter
Next up for Pompey is a trip to Oaklands Park to face Chichester City on Wednesday evening (7.45pm kick-off).
Tickets are available to purchase on the night and are priced £4 for adults and £1 for under 16s.