Pompey will face Norwich's youngsters in the group stage of the EFL Trophy.
The Premier League side were placed with the Blues, Crawley and Oxford in Friday's draw of invited under-21 clubs.
Kenny Jackett's side have two home group games in the competition, with the Canaries youngsters and League Two outfit Crawley visiting Fratton Park.
Pompey will also make the relatively short trip to fellow third tier side Oxford, with the dates and times of all matches to be confirmed.
Games are scheduled to be played in the weeks beginning September 2, October 7 and November 11, although there is some flexibility.
The 48 EFL clubs were placed into pre-determined groups, with consideration given to ensure travel time is minimised for supporters.
Southern Section
| Group A | Group B | Group C | Group D |
| Colchester | Crawley | AFC Wimbledon | Coventry |
| Gillingham | Norwich U21 | Brighton U21 | Forest Green |
| Ipswich | POMPEY | Leyton Orient | Southampton U21 |
| Tottenham U21 | Oxford | Southend | Walsall |
| Group E | Group F | Group G | Group H |
| Cheltenham | Bristol Rovers | Fulham U21 | Arsenal U21 |
| Exeter | Chelsea U21 | MK Dons | Cambridge |
| Newport | Plymouth | Stevenage | Northampton |
| West Ham U21 | Swindon | Wycombe | Peterborough |
Northern Section
| Group A | Group B | Group C | Group D |
| Grimsby | Accrington | Aston Villa U21 | Macclesfield |
| Leicester U21 | Fleetwood | Bury | Newcastle U21 |
| Scunthorpe | Liverpool U21 | Salford | Port Vale |
| Sunderland | Oldham | Tranmere | Shrewsbury |
| Group E | Group F | Group G | Group H |
| Burton | Bolton | Blackpool | Doncaster |
| Crewe | Bradford | Carlisle | Lincoln |
| Eveton U21 | Man City U21 | Morecambe | Man United U21 |
| Mansfield | Rochdale | Wolves U21 | Rotherham |