Tranmere will be looking to continue their renaissance next term and build on successive promotions.
They were preparing for a third campaign in the National League two years ago, but have since enjoyed a couple of play-off triumphs.
Pompey have not faced Rovers since the 2014/15 season, which saw the Merseyside outfit surrender the Football League status they had gained in 1921.
Tranmere finished sixth in League Two, but entered the play-offs having won just one of their final seven regular season games.
A 2-1 aggregate victory accounted for Forest Green in the semi-finals, however, before they struck right at the end of extra-time to beat Newport at Wembley.
Micky Mellon has been in charge at Prenton Park since October 2016 and guided Rovers to back-to-back promotions.
He previously took charge of Fleetwood and Shrewsbury, while his playing career included a couple of spells with Tranmere.
Full-back Adam Buxton made just four appearances during a short spell with Pompey under Paul Cook, but played a key role in last season’s promotion success.
Winger Ben Tollitt scored once in 18 games during his time at Fratton Park, although spent much of the 2018/19 campaign on loan at Wrexham.
HEAD TO HEAD
Pompey: 13 wins
Tranmere: 14 wins
Last Fratton meeting
Pompey 3 (Westcarr, Taylor 2)
Tranmere 2 (Myrie-Williams, Jones (og))
February 24 2015
Last away meeting
Tranmere 3 (Fenelon, Rowe, Power)
Pompey 1 (Westcarr)
November 29 2014
Best league finish: 4th in Division One (1992/93)
Ground: Prenton Park
Distance from Fratton Park: 260.4 miles
Estimated drive time: 4 hours 27 minutes
League One title odds: 50/1
2018/19 League Two: 6th
2017/18 National League: 2nd
2016/17 National League: 2nd
2015/16 National League: 6th
2014/15 League Two: 24th
2013/14 League One: 21st
2012/13 League One: 11th
2011/12 League One: 12th
2010/11 League One: 17th
2009/10 League One: 19th