Pompey Women will visit Maidenhead, Swindon, Stevenage and Brighton U23s this summer.
They also host Crystal Palace in a friendly at the PMC Stadium on August 11.
Maidenhead v Pompey: Sunday, July 7 (2pm kick-off)
The Blues kick-off their pre-season campaign with a trip to Maidenhead.
The Magpies currently compete one level lower down in the National League Division One South West.
They avoided relegation on the final day of last season, courtesy of a 1-0 victory at Southampton Saints.
Swindon v Pompey: Sunday, July 14 (2pm kick-off)
The second game on the calendar is a trip to Swindon, who are currently led by former development team assistant manager Dan Jones.
The sides last met during the 2017/18 campaign, where they faced off twice in quick succession.
Pompey ran out 5-0 winners in the home fixture – held at Bognor’s Nyewood Lane – with Daisy McLachlan and Samantha Quayle adding to Shannon Albuery’s hat-trick.
They then travelled to Cinder Lane seven days later and recorded a 3-0 victory, as McLachlan, Quayle and Katie James all found the target.
Swindon are also now competing in the South West division and finished in ninth position last season.
Stevenage v Pompey: Sunday, July 21 (2pm kick-off)
The Blues’ third summer opponents are currently placed in the South East section of National League Division One and also finished ninth.
They reached the Hertfordshire County Cup final, where they lost 4-0 to Watford’s under-23 side.
Brighton U23 v Pompey: Sunday, July 28 (kick-off TBA)
Pompey travel along the A27 to face south coast neighbours Brighton’s under-23 side in the final away friendly.
This was also the Blues’ concluding pre-season fixture last term and they fought from behind to win 3-2.
Brighton turned the game on its head after Carla Perkins’ long-range opener, only for a late Rachel Panting penalty and McLachlan wonder-strike to cap off a fine result.
Pompey v Crystal Palace: Sunday, August 11 (2pm kick-off)
Pompey end their preparations for the 2019/20 season by welcoming Championship outfit Crystal Palace to the PMC Stadium.
Their first campaign in the second tier saw Dean Davenport’s side finish in 10th spot, six points above basement club Millwall.
The Eagles secured three victories, seeing off London Bees, Millwall and Leicester on the road to earning 11 points.
A further friendly will take place on Sunday, August 4 and will be announced in due course.