It is a fixture that was played an incredible five times last season and there was very little to separate the sides.
Pompey’s highlight was their Wembley triumph in the EFL Trophy final, while Sunderland edged a tense play-off encounter.
And – just like last term – both sides are now firmly focused on the battle to win promotion to the Championship.
Kenny Jackett’s men climbed into the top six for the first time with victory at Lincoln on Tuesday evening, only to be pushed back out by the Black Cats 24 hours later.
The Wearsiders dismissed manager Jack Ross with the team occupying a play-off position in October and replacement Phil Parkinson was under pressure when they dropped as low as 15th.
But they have been in fine form in recent weeks and head to the south coast on a nine-match unbeaten run.
Unlike the Blues, Sunderland can also just focus on the league, having long since exited all the cup competitions.
They won 2-1 when the sides met in the north-east earlier in the campaign, battling from behind after Marcus Harness had opened the scoring.
Pompey have almost a fully-fit squad to choose from, with Bryn Morris (groin) the only player currently sidelined.
Duncan Watmore (leg) and Will Grigg (virus) missed Sunderland’s win at Tranmere on Wednesday evening.
The visitors have recently allowed out-of-favour winger Aiden McGeady to join Charlton on loan for the remainder of the campaign.
There have also been rumours of plenty of incomings and outgoings at the Stadum of Light before the transfer window closes at 11pm.
HEAD TO HEAD
Pompey: 34 wins
Sunderland: 39 wins
At Fratton Park
Pompey: 24 wins
Sunderland: 11 wins
Fratton Park is full to capacity for Saturday’s game.
Referee: Christopher Sarginson
Assistants: Michael Webb and Kevin Howick
Fourth Official: Gary Parsons
Overseas subscribers and match pass holders can watch the action and commentary will begin from around 10 minutes before kick-off.
ALSO IN LEAGUE ONE
All games kick-off at 3pm
Accrington v AFC Wimbledon
Bolton v Tranmere
Bristol Rovers v Coventry
Fleetwood v Doncaster
Ipswich v Peterborough
MK Dons v Wycombe
Oxford v Blackpool
Rochdale v Shrewsbury
Rotherham v Burton
Southend v Lincoln