Pompey Women face the UK Armed Forces representative squad at HMS Temeraire's Victory Stadium next Thursday (January 9).
The mid-season friendly gives Jay Sadler's squad a chance to get more minutes under their belt as they head into a busy second half of the season.
Their opponents will pick players from the Royal Navy, British Army and Royal Air Force to face Pompey.
Blues boss Jay Sadler said: "Portsmouth is a city with rich Naval history, so as soon as the Armed Forces side contacted us, it was a no-brainer.
"Portsmouth is a city with rich naval history, so as soon as the Armed Forces side contacted us, it was a no-brainer."
"Not only is this a fantastic opportunity to link with their set-up, but it also gives us a great opportunity to implement some new ideas mid-season.
"We have players who are in need of minutes, as well as a few younger players impressing within our set-up. This game provides us with a great opportunity to do this."
The clash – which kicks off at 7.45pm – is open to spectators and entry is free.