Pompey had to settle for runners-up spot in Southern Group A of the Papa John's Trophy.
They were beaten 1-0 by West Ham U21s at Fratton Park and will now be on the road in the second round.
Kenny Jackett named a young side against the Hammers, with six players – including Harry Kavanagh – making their debuts.
Fans can watch the key moments (top video) or register with iFollow for extended highlights (middle video) and a full match replay (bottom video).