A penalty ensured that Pompey Academy suffered their first defeat of the new season, as Bristol Rovers left Furze Lane with all three points.
Matt Reed converted midway through the first half after Issiaga Kaba was adjudged to have committed a foul.
The young Blues had a late chance to level when Seok Jae Lee (pictured) was brought down in the box, but Harrison Brook’s spot-kick was saved by Jed Ward.
Professional development phase coach Liam Daish said: “We started fairly well, but then started to get frustrated when things weren’t working for us.
“Bristol Rovers scored their penalty, then defended very deep and were looking to hit us on the counter.
“We struggled to identify where the space was to attack them and probably played into their hands a little bit.
"The more concerning thing for me was that 20-minute period when we didn’t react well enough to things not coming off."
“Our attitude was much better after the break and we didn’t let any feelings of disappointment last too long.
“We moved the ball well and created some opportunities, but couldn’t convert the penalty we were awarded and that lifted Rovers’ spirits.
“When you look at the amount of possession we had, we deserved more, although didn’t really create enough clear-cut chances.
“The more concerning thing for me was that 20-minute period when we didn’t react well enough to things not coming off. We need to handle it better in those situations.”
Pompey: Pitman; Setters (Hughes 70), Kaba, Cadman, Rew; Jewitt-White, Bell; Lee, Dawson (Bridgman 56), Brook; Anderson