Pompey dropped out of the League One play-off places after being narrowly beaten at MK Dons.
The 1-0 defeat – coupled with results elsewhere – saw them slip to seventh position with five games of the campaign still to play.
Scott Fraser netted only goal from the penalty spot, although Danny Cowley's men had plenty of chances in an open game, with Tom Naylor hitting the crossbar before Jack Whatmough was sent off late on.
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