Pompey Women’s FA Cup run came to an end with an extra-time defeat to Southampton at Westleigh Park.
Cherelle Khassal had ensured the hosts led at the interval, but Lucia Kendall levelled after the break before, with a penalty shoot-out looming, Caitlin Morris struck the winner.
It was the first of two meetings in the space of four days between the south coast rivals, with a league contest taking place at Fratton Park on Wednesday night.
Jay Sadler made two changes from the side that eased to victory over QPR in the National League Plate the previous week.
Evie Gane and Sammy Quayle both returned to the starting line-up, as Rebekah Tonks and Rosie McDonnell dropped to the bench.
Pompey started brightly and a delivery towards the back post by Quayle was agonisingly out of reach for Shannon Albuery.
Kayla Rendell then had to dive at the feet of Khassal after Hayley Bridge had threaded a pass through for the striker.
They kept pushing in search of an opener and Freya Jones fired wide after the ball landed to her from a corner.
Rendell was then called into action again, keeping out a Khassal half-volley following good work from Danielle Rowe.
But the Blues did make their pressure count on 31 minutes, as a neat Albuery flick found KHASSAL, who smashed the ball into the top corner.
The lead was almost doubled just seconds later, but Rendell managed to tip Albuery’s header over the bar.
And the home side were left fuming when Quayle appeared to be dragged down inside the area, only for the referee to remain unmoved.
Half Time: Pompey 1 Southampton 0
The second half almost got off to the perfect start when Albuery raced away, but again Rendell came to the visitors’ rescue.
Pompey kept sending crosses into the box as they looked for a cushion, with Southampton being placed under plenty of pressure.
But they netted an equaliser just past the hour mark, as Kendall found space to head past Hannah Haughton.
The Blues tried to recover from that setback and the ball narrowly missed Quayle when Khassal flashed a low delivery across the face of goal.
Appeals for a handball on the line were then dismissed before an attempt from Khassal was deflected past the post.
And with neither side able to create any further meaningful chances, an additional 30 minutes would be required.
Full Time: Pompey 1 Southampton 1
It proved to be particularly cagey when the action restarted, with both sides understandably keen to stay strong at the back.
But it was Southampton who took the lead midway through the second period of extra-time, as a corner was bundled home by Morris.
The hosts tried their best to find a late equaliser and take the tie to penalties, but their opponents held firm to progress into round four.
Pompey: Haughton; Rowe (c), Gotch, Gane; Rowbotham (Younger 114); Bridge, F.Jones (Tonks 100), Lane, Quayle (McDonnell 83); Khassal, Albuery
Subs not used: Kingshott, Stroem, Laidlaw, Cleeve, Walters, Aldridge