It is a clash between third and first at Fratton Park this weekend, with Hull the visitors to the south coast.
And victory for Pompey could see them replace their opponents at the top of the League One standings.
They have already enjoyed one success over the Tigers this term, with a 2-0 win at the KCOM Stadium just before Christmas.
But Kenny Jackett knows that his side have to focus on more than just winning one fixture against fellow promotion hopefuls.
The Blues boss said: “Of course it’s a big game and it’s always great to be able to affect the sides around or above you in the table.
“But just winning this one isn’t going to get us to where we want to be – we have to prove ourselves on a consistent basis.
“If you’re one of those three sides that secure promotion at the end of the season, then you’ll need a big points total.
"Just winning this one isn’t going to get us to where we want to be – we have to prove ourselves on a consistent basis."
“We’ve shown with our results already this season that we’re capable of competing with every team in our division.
“It’s not about individual matches, but this one is next up for us, so we’re fully focused on it and looking to continue building momentum.
“Hull came out of the Championship last season and will be aiming to go straight back up with a strong squad.
“There are players with a good level of experience and those who have played at a higher level. We’ll respect them, but are looking forward to the test.”
Pompey’s home would, in normal circumstances, be packed for such a mouth-watering contest, but the threat of Covid-19 means games are likely to take place behind closed doors for some time yet.
Jackett said: “It’s a shame that supporters are not going to be there because we know they would produce an excellent atmosphere.